Volume 86, Issue 1, July (2001), pp. 31-36 © The Author 2001

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High fat intake lowers hepatic fatty acid synthesis and raises fatty acid oxidation in aerobic muscle in Shetland ponies

Suzanne N. J. Geelen¹*, Cristina Blázquez², Math J. H. Geelen³, Marianne M. Sloet van Oldruitenborgh-Oosterbaan¹, and Anton C. Beynen^4
1Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
²Department of Biochemistry and Molecular Biology I, Faculty of Biology, Complutense University, Madrid, Spain
³Laboratory of Veterinary Biochemistry, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
⁴Department of Nutrition, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands

 (Received 3 August 2000–Revised 27 November 2000–Accepted 15 January 2001)

The metabolic effects of feeding soyabean oil instead of an isoenergetic amount of maize starch plus glucose were studied in ponies. Twelve adult Shetland ponies were given a control diet (15 g fat/kg DM) or a high-fat diet (118 g fat/kg DM) according to a parallel design. The diets were fed for 45 d. Plasma triacylglycerol (TAG) concentrations decreased by 55 % following fat supplementation. Fat feeding also reduced glycogen concentrations significantly by up to 65 % in masseter, gluteus and semitendinosus muscles (P<0·05, P<0·01 and P<0·01 respectively). The high-fat diet significantly increased the TAG content of semitendinosus muscle by 80 % (P<0·05). Hepatic acetyl-CoA carboxylase and fatty acid synthase activities were 53 % (P<0·01) and 56 % (P<0·01) lower respectively in the high-fat group, but diacylglycerol acyltransferase activity was unaffected. Although carnitine palmitoyltransferase-I (CPT-I) activity in liver mitochondria was not influenced, fat supplementation did render CPT-I less sensitive to inhibition by malonyl-CoA. There was no significant effect of diet on the activity of phosphofructokinase in the different muscles. The activity of citrate synthase was raised significantly (by 25 %; P<0·05) in the masseter muscle of fat-fed ponies, as was CPT-I activity (by 46 %; P<0·01). We conclude that fat feeding enhances both the transport of fatty acids through the mitochondrial inner membrane and the oxidative capacity of highly-aerobic muscles. The higher oxidative ability together with the depressed rate of de novo fatty acid synthesis in liver may contribute to the dietary fat-induced decrease in plasma TAG concentrations in equines.

Keywords:
Equine: Dietary fat: Hepatic lipogenesis: Muscle fat metabolism: Fatty acid oxidation