Volume 89, Issue 3, March (2003), pp. 351-358 © The Author 2003
doi:10.1079/BJN2002803

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Detection of caseinophosphopeptides in the distal ileostomy fluid of human subjects

H. Meisel^1,CA, H. Bernard², S. Fairweather-Tait³, R. J. FitzGerald⁴, R. Hartmann¹, C. N. Lane⁵, D. McDonagh⁵, B. Teucher³ and J. M. Wal^2
1Federal Dairy Research Centre, Institute for Dairy Chemistry and Technology, Kiel, Germany
²Laboratoire d'Immuno-Allergie Alimentaire, INRA-CEA, SPI, Gif sur Yvette, France
³Institute of Food Research, Norwich Research Park, Norfolk, UK
⁴University of Limerick, Life Sciences Department, Limerick, Republic of Ireland
⁵Teagasc, Dairy Products Research Centre, Moorepark, Fermoy, Co. Cork, Republic of Ireland

 (Received 23 April 2002–Revised 16 October 2002–Accepted 31 October 2002)

Caseinophosphopeptides (CPP) were detected for the first time in ileostomy fluid, collected at 2h intervals for 10h post milk and CPP ingestion, from human volunteers with an ileostomy. The level of CPP present in ileostomy fluid obtained from milk-fed volunteers was markedly higher than that from volunteers fed with selected CPP preparations. The findings are based on HPLC analysis in combination with peptide-bound P determination, thin-layer electrophoresis and amino acid analysis, together with ELISA studies using polyclonal antibodies raised against a set of CPP to detect immunoreactive CPP in ileostomy fluid. These procedures allowed the detection of nM concentrations of CPP. CPP, which can be released during intestinal digestion, may function as bioactive constituents and carriers for different minerals, especially Ca, and may be used as ingredients in functional foods or pharmaceutical preparations.

Abbreviations: CCP; caseinophosphopeptide; EIA; enzyme immunoassay; SCX; strong cation exchange; SerP; phosphoserine

Keywords:
Caseinophosphopeptides, Milk, Human ileostomy samples, Bioactive peptides