Volume 90, Issue 2, August (2003), pp. 353-362 © The Author 2003
doi:10.1079/BJN2003907

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Use of biomarkers of sub-clinical infection, nutrition and neonatal maturity to interpret plasma retinol in Nigerian neonates

Delana A. Adelekan¹, Christine A. Northrop-Clewes³, Joshua A. Owa², Adesola O. Oyedeji⁴, Adedayo A. Owoeye⁴, David I. Thurnham^3
1Department of Community Health and
²Department of Paediatrics and Child Health, Obafemi Awolowo, University Ile-Ife, Nigeria
³Northern Ireland Centre for Diet and Health (NICHE), University of Ulster, Coleraine BT52 1SA, Northern Ireland, UK
⁴Department of Paediatrics, Wesley Guild Hospital, Ilesa, Nigeria

 (Received 26 June 2002–Revised 17 March 2003–Accepted 9 April 2003)

Using the World Health Organization criterion, the prevalence of sub-clinical vitamin A deficiency can be assessed using plasma retinol concentrations <0·7 μmol/l. However, plasma retinol can be depressed by infection; thus, the use of this criterion alone may overestimate deficiency. In the present study, we investigated the usefulness of the acute-phase proteins (APP) α₁-antichymotrypsin (ACT) and α₁-acid glycoprotein (AGP), plasma carotenoids and anthropometric and gestational indices to interpret plasma retinol in the blood of 192 apparently healthy Nigerian neonates collected randomly during days 1–20 postpartum. The mean weight (2·64 kg) and length (0·458 m) of the neonates and plasma concentrations (geometric mean, μmol/l) of retinol (0·54), α-carotene (0·072), ß-carotene (0·076) and lutein (0·080) were low. The prevalence of vitamin A deficiency was 72 %, indicating a severe public health problem. Babies who were of low birth weight (P<0·003) or premature and low birth weight (P<0·023) had significantly lower retinol concentrations than full-term normal weight babies. Thirty-two neonates had abnormal ACT and forty-four abnormal AGP concentrations. Positive correlations between retinol and ACT (r 0·186, P=0·05) and AGP (r 0·31, P=0·0001) during days 1–5 may be due to the increasing plasma retinol from maternal milk and a coincidental increasing capacity to synthesise APP. Subsequently, negative correlations between retinol and ACT (r −0·28, P=0·02) and AGP (r −0·29, P=0·018) from day 6 onwards reflected the continuing increase in plasma retinol, but no further increase in the APP. Overall, weight, ACT, lutein and age explained 30 % of the variance in retinol, but lutein was the most significant (r² 0·18, P<0·0001). Hence, the distribution of plasma retinol concentrations in this group of neonates was more strongly linked with nutrition (via the surrogate marker lutein) than infection.

Keywords:
Retinol, Lutein, Acute-phase proteins, α₁-Antichymotrypsin, α₁-Acid glycoprotein, Nigeria, Neonate

Abbreviations:
ACT, α₁-antichymotrypsin, AGP, α₁-acid glycoprotein, APP, acute-phase protein, VAD, vitamin A deficiency