Volume 91, Issue 1, January (2004), pp. 101-106 © The Author 2004
doi:10.1079/BJN20031025

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Oxyphytosterols are present in plasma of healthy human subjects

André Grandgirard¹, Lucy Martine¹, Luc Demaison¹, Catherine Cordelet¹, Corinne Joffre¹, Olivier Berdeaux¹, Etienne Semon^2
1Unité de Nutrition Lipidique, INRA, 17 rue Sully, BP 86510, 21065 , DIJON cedex, France
²Laboratoire de Recherches sur les Arômes, INRA, Dijon, France

 (Received 5 June 2003–Revised 15 September 2003–Accepted 16 September 2003)

The oxidised derivatives of phytosterols (oxyphytosterols) were identified in plasma samples from thirteen healthy human volunteers, using MS. All the samples contained noticeable quantities of (24R)-5β,6β-epoxy-24-ethylcholestan-3β-ol (β-epoxysitostanol) and (24R)-ethylcholestan-3β,5α,6β-triol (sitostanetriol) and also trace levels of (24R)-5α,6α-epoxy-24-ethylcholestan-3β-ol (α-epoxysitostanol), (24R)-methylcholestan-3β,5α,6β-triol (campestanetriol) and (24R)-ethylch olest-5-en-3β-ol-7-one(7-ketositosterol). The amounts of these oxyphytosterols in plasma varied from 4·8 to 57·2 ng/ml. There are two possibilities concerning the origin of these compounds. First, they could come from the small amounts of oxyphytosterols in food. Second, they could originate from the in vivo oxidation of phytosterols in plasma. Very few data actually exist concerning these compounds. Their identification in human samples suggests that further research is necessary in this field.

Keywords:
Oxyphytosterols, Phytosterols, Human plasma, Mass spectrometry

Abbreviations:
Campestanetriol, (24R)-methylcholestan-3β,5α,6β-triol, α-epoxysitostanol, (24R)-5α,6α-epoxy-24-ethylcholestan-3β-ol, β-epoxysitostanol, (24R)-5β,6β-epoxy-24-ethylcholestan-3β-ol, 19-hydroxycholesterol, cholest-5-en-3β,19-diol, 27-hydroxycholesterol, (25R)-cholest-5-en-3β,26-diol, 7-ketositosterol, (24R)-ethylcholest-5-en-3β-ol-7-one, sitostanetriol, (24R)-ethylcholestan-3β,5α,6β-triol, TBME, tert-butyl methyl ether, TMSE, trimethylsilyl ether, TMSOH, hydroxytrimethylsilyl fragment